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GA attenuated the LPS-induced M1 polarization of RAW264.7 cells. (A) Fluorescence intensity of M1-polarized macrophages (CD32) measured by flow cytometry. (B) Fluorescence intensity of M2-polarized macrophages (CD206) measured by flow cytometry. (C–F) Relative expression levels of M1 markers (IL-1β, TNF-α, iNOS, and CD32). (G) Relative expression levels of M2 markers (CD206). (H) mRNA level of NOX4. (I) Western blots (IL-1β, TNF-α, IBA-1, COX2, NOX4, Nrf2, p-Nrf2). # compared with the control group. *compared with the LPS group. p < 0.05.

Journal: Frontiers in Immunology

Article Title: Gallic acid exerts therapeutic effects on sciatica by reducing inflammatory responses through the regulation of NOX4-mediated oxidative stress

doi: 10.3389/fimmu.2026.1748652

Figure Lengend Snippet: GA attenuated the LPS-induced M1 polarization of RAW264.7 cells. (A) Fluorescence intensity of M1-polarized macrophages (CD32) measured by flow cytometry. (B) Fluorescence intensity of M2-polarized macrophages (CD206) measured by flow cytometry. (C–F) Relative expression levels of M1 markers (IL-1β, TNF-α, iNOS, and CD32). (G) Relative expression levels of M2 markers (CD206). (H) mRNA level of NOX4. (I) Western blots (IL-1β, TNF-α, IBA-1, COX2, NOX4, Nrf2, p-Nrf2). # compared with the control group. *compared with the LPS group. p < 0.05.

Article Snippet: The antibodies used were as follows were as follows: PE anti-mouse CD32 Antibody (156404, Biolegend), APC anti-mouse CD206 Antibody (141708, Biolegend), anti-COX2 (WL01750, Wanleibio), anti-iNOS (2982S, CST), anti-NOX4 (ZENBIO,380874), anti-ATF4(ET1612-37, HUABIO), anti-IBA-1(Bimake, A5595), anti-TNF-α(ZENBIO, 346654), anti-Nrf2 (Affinity,BF8017), anti-p-Nrf2 (Affinity,DF7519), anti-IL1β (Wanlei-Bio,WL00891), and anti-β-actin (ZENBIO, 380624).

Techniques: Fluorescence, Flow Cytometry, Expressing, Western Blot, Control

GA affected the activation of M1/M2 macrophages. (A, B) Coexpression analysis of IBA-1/CD32 and IBA-1/CD206. # Compared with the sham operation group; *compared with the CCI group; p < 0.05.

Journal: Frontiers in Immunology

Article Title: Gallic acid exerts therapeutic effects on sciatica by reducing inflammatory responses through the regulation of NOX4-mediated oxidative stress

doi: 10.3389/fimmu.2026.1748652

Figure Lengend Snippet: GA affected the activation of M1/M2 macrophages. (A, B) Coexpression analysis of IBA-1/CD32 and IBA-1/CD206. # Compared with the sham operation group; *compared with the CCI group; p < 0.05.

Article Snippet: The antibodies used were as follows were as follows: PE anti-mouse CD32 Antibody (156404, Biolegend), APC anti-mouse CD206 Antibody (141708, Biolegend), anti-COX2 (WL01750, Wanleibio), anti-iNOS (2982S, CST), anti-NOX4 (ZENBIO,380874), anti-ATF4(ET1612-37, HUABIO), anti-IBA-1(Bimake, A5595), anti-TNF-α(ZENBIO, 346654), anti-Nrf2 (Affinity,BF8017), anti-p-Nrf2 (Affinity,DF7519), anti-IL1β (Wanlei-Bio,WL00891), and anti-β-actin (ZENBIO, 380624).

Techniques: Activation Assay